Journal: Applied and Environmental Microbiology

Article Title: Heterologous Overexpression and Characterization of a Flavoprotein-Cytochrome c Complex Fructose Dehydrogenase of Gluconobacter japonicus NBRC3260

doi: 10.1128/AEM.03152-12

Figure Lengend Snippet: Bacterial strains and plasmids used in this study

Article Snippet: Kanamycin and ampicillin were used at final concentrations of 50 μg ml −1 and 250 μg ml −1 , respectively. table ft1 table-wrap mode="anchored" t5 caption a7 Strain or plasmid Description Source or reference a Strains Escherichia coli DH5α F − endA1 hsdR17 (r k − m k + ) supE44 thi-1 λ − recAl gyrA96 relA1 deoR Δ( lacZYA-argF )U169 φ80d lacZ ΔM15 10 HB101 F − thi-1 hsdS20 (r B m B ) supE44 recA13 ara14 leuB6 proA2 lacY1 galK2 rpsL20 (Str r xyl-5 mtl-1 λ − ) 34 Gluconobacter japonicus NBRC3260 Wild type NBRC Gluconobacter oxydans NBRC12528 Wild type NBRC Δ adhA mutant NBRC12528 Δ adhA ::Km r 9 ATCC 621H Wild type ATCC Plasmids pKR2013 Plasmid mediates plasmid transfer; Km r 11 pBBR1MCS-4 Broad-host-range plasmid; mob Ap r 35 pSHO8 pBBR1MCS-4, a 0.7-kb fragment of a putative promoter region of the adhAB gene of G. oxydans 621H This study pSHO12 pSHO8, a 3.8-kb fragment of the fdhSCL genes of G. japonicus NBRC3260 This study pSHO13 pSHO8, a 3.7-kb fragment of the fdh ATG SCL genes This study pSHO16 pSHO8, a 2.4-kb fragment of the fdh ATG SL genes (in-frame deletion of fdhC ) This study Open in a separate window a The URL addresses of NBRC and ATCC are http://www.nbrc.nite.go.jp/ and http://www.atcc.org/ , respectively.

Techniques: Plasmid Preparation, Mutagenesis

Journal: Molecular Cell

Article Title: Impact of chromatin context on Cas9-induced DNA double-strand break repair pathway balance

doi: 10.1016/j.molcel.2021.03.032

Figure Lengend Snippet:

Article Snippet: CloneCatcher DH5α electrocompetent E. coli , Genlantis , Cat# C810111.

Techniques: Western Blot, Virus, Recombinant, Transfection, Lysis, Viability Assay, Protease Inhibitor, Reverse Transcription, Plasmid Preparation, Software